Research Network for Metals in Medicine

 

 

Dr Debbie Trinder

Position: Research Fellow

Affiliation: Iron Research Group, Fremantle Hospital

Postal Address:
Department of Medicine
University of Western Australia
Level 7, Education Centre
Fremantle Hospital
Attfield Street
Fremantle
Western Australia 6160
AUSTRALIA

Phone: +61 (08) 9431 3640
Email: dtrinder@cyllene.uwa.edu.au
Webpage: http://www.meddent.uwa.edu.au/medicine/index.htm


Research Profile

Dr Debbie Trinder has been a Research Fellow at the University of Western Australia since 1995. Her major area of research is iron metabolism. It has been the focus of her PhD studies at UWA, postdoctoral studies in USA and on going research at UWA. During this period she has published 35 papers in international peer reviewed journal including a number of publications in Proceeding of National Academy of Science USA, Journal of Biological Chemistry, Hepatology, Gut ad American journal of Physiology. She has also written invited book chapter and 3 invited review articles on Iron Metabolism. DT was the recipient of research awards sincluding NHMRC CJ Martin Fellowship (1992-1996) and Raine Medical Research Foundations Fellowship (1997-1998). DT has been chief investigator on a number of research grants receiving over $1.5 million funding from NHMRC, ARC, Ramaciotti Research Foundation, Western Australian Institute for Medical Research, Fremantle Hospital Medical Research Foundation and Lotteries Commission of WA. She has made many presentations at national and international meetings including invited presentations at the following international meetings: International Symposium on Iron in Biology and Medicine, Saint Malo, France (1997), Pacific Associate for the Study of Liver Disease, Perth, Australia (1998), Symposium on Iron Transport, 5th Internet World Congress on Biomedica Science, Montreal, Canada (1998), Symposium on iron metabolism 6th Internet World Congress for Biomedical Sciences, Spain (2000), BioIron, world Congress for the study of Disorders of Iron Metabolism, Cairns, Australia (2001), BioIron, World Congress for the study of Disorders of Iron Metabolism, Washington, USA (2003) and Digestive Disease Week, Orlando, Florida, USA (2003). DT is a participate in the Western Australian Institute for Medical Research and is currently supervising 2 PhD in collaboration with Prof John Olynyk and Dr Phil Oates. She has undertaken teaching to undergraduate Science and Medical students at UWA since 1996.

The main focus of the research of our group is iron metabolism in health and disease. I have developed a laboratory based research program investigating the molecular mechanisms in normal iron metabolism and in the development of iron overload disorder hereditary haemochromatosis. We use transgenic murine models of iron overload and tumor cell lines to investigate cellular iron transport using radiolabelled tracers and expression of iron transporters and regulators of iron homeostasis. We have expertise in the area of molecular and cellular biology of iron including cell isolation and culture, iron transport, gene and protein expression, gene knockdown and immunohistochemistry. Our experience in these areas should be useful for participants in the ARC Metals in Medicine Network for the testing or screening of metals or metal complexes in biological systems.

Selected Publications

  1. 1. D. Trinder, E.H. Morgan and E. Baker. The mechanisms of iron uptake by fetal hepatocytes in culture. Hepatology 1986, 6:852-858.
  2. D. Trinder, E.H. Morgan and E. Baker. The effects of an antibody to the rat transferrin receptor and rat serum albumin on the uptake of diferric transferrin by rat hepatocytes. Biochima Biophysica Acta 1988, 943:440-446.
  3. E. Baker, D. Trinder, J. Torrance, M. Page, E.H. Morgan (1988) Characteristics of iron exchange between transferrin an hepatocytes in culture in "Thalassaemia: Pathophysiology and Management" (March of Dimes Foundation N.Y.).
  4. D. Trinder, R.G. Batey, E.H. Morgan and E.Baker. The effect of the cellular iron concentration on the uptake of iron by rat hepatocytes. American Journal of Physiology 1990, 259:G611-G617.
  5. O. Zak, D. Trinder and P. Aisen Primary Recpetor-Recognition Site of Human Transferrin is in the C-terminal lobe. Journal of Biology Chemistry 1994, 269:7110-7114.
  6. K. Thorstensen, D. Trinder, O. Zak and P. Aisen Uptake of iron from N-terminal half-transferrin by isolated rat hepatocytes: evidence of transferrin receptor-independent iron uptake. European Journal of Biochemistry 1995,232:129-133.
  7. D. Trinder, O. Zak and P. Aisen. Transferrin receptor independent uptake of diferric transferrin by human hepatoma cells with antisense inhibition of receptor expression. Hepatology 1996, 23:1512-1520.
  8. D. Trinder and E.H. Morgan. Inhibition of uptake of transferrin-bound iron by human hepatoma cells by nontransferrin-bound iron. Hepatology 1997, 26:691-698.
  9. D. Trinder and E.H. Morgan. (1998) Mechanisms of ferric citrate uptake by human hepatoma cells. American Journal of Physiology 275 (Gastrointest. Liver Physiol. 38) G279-286
  10. D. Trinder, P.S. Oates, C. Thomas, J.Sadleir and E.H. Morgan. (1998) The expression and distribution of rat Nramp2. Symposium on Iron Metabolism, 5th Internet World Congress on Biomedical Sciences at, McMaster University, Canada. http://www.mcmaster.ca/inabis98/.
  11. D. Trinder, P.S. Oates, C. Thomas, J.Sadleir and E.H. Morgan. Localisation of Nramp2 to the microvillus membrane of rat duodenal enterocytes in iron deficiency but of hepatocytes in iron overload. Gut 2000, 46:270-276.
  12. E. Baker, D.Trinder, P. Oates, Thomas and E. Morgan (2000) Transferrin-bound and Non-transferrin bound iron uptake by mammalian cells. Symposium on Iron Metabolism 6th Internet World Congress for Biomedical Sciences at, Castilla-La Mancha University, Spain. http://www.uclm.es/inabis2000.
  13. T. Moos, D. Trinder and E.H. Morgan. Cellular distribution of ferric iron, ferritin, transferrin and divalent metal transporter (DMT1) in substantia nigra and basal ganglia of the normal and ?2 microglobulin knockout mouse brain. Cellular and Molecular Biology 2000, 46:549-561.
  14. D. Trinder, D. Macey and J.K. Olynyk. The new iron age. International Journal of Molecular Medicine 2000, 6, 607-612.
  15. P.S. Oates, D. Trinder and E.H. Morgan Divalent metal transporter activity and intestinal iron absorption. Pflugers Archives European Journal of Physiology. 2000, 440:496-502 Frazier D, Vulpe C, McKie A, Wilkins S, Trinder D, Cleghorn CJ and G Anderson. Cloning and gastrointestinal expression of rat hephaestin: relationship to other iron transport proteins American Journal of Physiology 2001, 281:G931-G939.
  16. D. Trinder, J.K. Olynyk, W.S. Sly, E.H. Morgan. Iron uptake from plasma transferring by the duodenum is impaired in the HFE knockout mice. Proceedings of the National Academy of Science (USA). 2002,99:5622-5626.
  17. D. Trinder, C. Fox, G. Vautier and JK Olynyk. Molecular pathogenesis of iron overload. Gut 2002,51:290-295.
  18. D. Trinder and E.H. Morgan (2002) Uptake of transferrin-bound iron by mammalian cells In: “Molecular and Cellular Iron Transport” pp 427-449. eds. D. Templeton. Marcel Dekker, NY.
  19. T. Moos, D. Trinder and E.H. Morgan. The effect of iron status on divalent metal transporter 1 (DMT1) expression in duodenal enterocyte from the ß2-microglobulin knockout mouse. American Journal of Physiology 2002, 283:G687-G694.
  20. D. M. Frazier, S.J. Wilkins, E.M. Becker, C.D. Vulpe, A.T. McKie, D. Trinder and G.J. Anderson. Hepicidin expression inversely correlates with expression of duodenal iron transporters and iron absorption in rats. Gastroenterology 2002, 123:835-
  21. A. Lee, P.S. Oates and D. Trinder Effects of cell proliferation on the uptake of transferrin-bound iron by hepatoma cells Hepatology 2003,38:967-977.
  22. D. Trinder and E. Baker Transferrin receptor two: a new molecule in iron metabolism International Journal of Biochemistry and Cellular Biology 2003,35,292-296.

Facilities

Iron Research Group is part of the School of Medicine and Pharmacology at Fremantle Hospital. The School has new PC2 research laboratories that are well equipped to undertake molecular and cellular biology research with cell culture suites, a radioisotope laboratory, PCR and protein laboratories and small animal surgical room and a new on-site animal facility.

International Linkages

Professor Robert Britton (St. Louis University)
Professor William Sly (St. Louis University)
Associate Professor Robert Fleming (St. Louis University)
Associate Professor Torben Moos (University of Copenhagen)
Professor Philip Aisen. (Albert Einstein College of Medicine)