Research Network for Metals in Medicine

 

 

Dr Luis Filgueira

Position: Senior Lecturer

Affiliation: University of Western Australia, School of Anatomy & Human Biology

Postal Address:
The School of Anatomy & Human Biology
The Faculty of Life and Physical Sciences
The University of Western Australia
35 Stirling Highway
CRAWLEY WA 6009
AUSTRALIA

Phone: +61 (08) 6488 3907
Email: lfilgueira@anhb.uwa.edu.au
Webpage: http://school.anhb.uwa.edu.au/personalpages/filgueira/default_files/slide0001.htm


Research Profile

Main expertise in immunology and morphology:
Cellular immunology, including cell culture and functional tests using human, mouse and rat in vitro models (proliferation assays; antigen presentation assays; generation of antigen-specific T-cells in vitro; generation of antigen presenting cells, including EBV transformed B-lymphocytes and dendritic cells, flow cytometry, ELISA, RT-PCR). More recently, working on metals as antigens: uptake, processing and presentation.

Morphology: light, fluorescence and electron microscopy for biological samples (standard methods; immune labelling methods; molecule and particle tracking methods; x-ray analysis). More recently, exploring methods for investigating metal processing by immune cells and osteoclasts.

Selected Publications

  1. L Filgueira, P Groscurth, M Aguet. Binding and internalization of gold-labelled IFN-g by human Raji cells. J. Immunol. 1989, 142, 3436.
  2. L Filgueira, M Zuber, A Merlo, F Harder, M Heberer, GC Spagnoli.. Effects of different culture protocols on the expression of discrete T cell receptor variable regions in human tumor infiltrating lymphocytes. Eur. J. Cancer 1993, 29A, 1754.
  3. L Filgueira, V Caetano, U Lüscher, A Juretic, M Zuber, F Harder, G Garotta, M Heberer, GC Spagnoli.. Differential effects of interleukin-2 and CD3 triggering on cytokine gene transcription and secretion in cultured human infiltrating lymphocytes. Cell. Immunol. 1993,150, 205.
  4. L Filgueira, FO Nestlé, M Rittig, HI Joller, P Groscurth. Human dendritic cells phagocytose and process Borrelia burgdorferi. J. Immunol. 1996, 157, 2998.
  5. G Grouard, I Durand, L Filgueira, J Banchereau, A-J Liu. Dendritic cells capable of stimulating T cells in germinal centres. Nature 1996, 383,364.
  6. G Grouard, M-C Rissoan, L Filgueira, I Durand, J Banchereau, Y-J Liu. The enigmatic plasmocytoid T cells deveop into dendritic cells with IL-3 and CD40-ligand. J. Exp. Med. 1997, 185, 1101.
  7. FO Nestlé, L Filgueira, BJ Nickoloff, G Burg. Human dermal dendritic cells process and present soluble protein antigens. J. Investig. Dermatol. 1998, 110, 762.
  8. Ch Beermann, G Lochnit, R Geyer, P Groscurth, L Filgueira. The lipid-component of lipoproteins from Borrelia burgdorferi: structural analysis, antigenicity and presentation via human dendritic cells. Biochem. Biophys. Res. Commun. 2000, 267, 897.
  9. Ch Beermann, H Wunderli-Allenspach, P. Groscurth, L Filgueira. Lipoproteins from Borrelia burgdorferi applied in liposomes and presented by dendritic cells induce CD8+ T-lymphocytes in vitro Cell. Immunol. 2000, 201, 124-131.
  10. SJ Wu, G Grouard, JR Mascola, R Putvana, MK Louder, L Filgueira, M Marovich, E Brachtel, G Murphy, N Bhamapravati, DL Birx, C Hayes, S Schlesinger Frankel. Human skin Langerhans cells are targets in transmission of dengue virus infection. Nature Medicine 2000, 6, 816-820.
  11. R Nunez, L Filgueira. Flow cytometric assessment of the reactivity of a panel of monoclonal antibodies (mAb) against two populations of human dendritic cells (DC). BMC Immunology 2001, 2:6 http://www.biomedcentral.com/1471-2172/2/6
  12. E Eppler, H Horig, HL Kaufman, P Groscurth, L Filgueira. Carcinoembryonic antigen (CEA) presentation and specific T cell-priming by human dendritic cells transfected with CEA-mRNA. European Journal of Cancer 2002, 38, 184-193.
  13. L Filgueira. Fluorescence-based staining for tartrate-resistant acidic phosphatase (TRAP) in osteoclasts combined with other fluorescent dyes and protocols. J. Histochem. Cytochem. 2004, 52, 411.

Facilities

Infrastructure for processing of biological samples for light and electron microscopy.