Research Network for Metals in Medicine



Dr Luis Filgueira

Position: Senior Lecturer

Affiliation: University of Western Australia, School of Anatomy & Human Biology

Postal Address:
The School of Anatomy & Human Biology
The Faculty of Life and Physical Sciences
The University of Western Australia
35 Stirling Highway

Phone: +61 (08) 6488 3907

Research Profile

Main expertise in immunology and morphology:
Cellular immunology, including cell culture and functional tests using human, mouse and rat in vitro models (proliferation assays; antigen presentation assays; generation of antigen-specific T-cells in vitro; generation of antigen presenting cells, including EBV transformed B-lymphocytes and dendritic cells, flow cytometry, ELISA, RT-PCR). More recently, working on metals as antigens: uptake, processing and presentation.

Morphology: light, fluorescence and electron microscopy for biological samples (standard methods; immune labelling methods; molecule and particle tracking methods; x-ray analysis). More recently, exploring methods for investigating metal processing by immune cells and osteoclasts.

Selected Publications

  1. L Filgueira, P Groscurth, M Aguet. Binding and internalization of gold-labelled IFN-g by human Raji cells. J. Immunol. 1989, 142, 3436.
  2. L Filgueira, M Zuber, A Merlo, F Harder, M Heberer, GC Spagnoli.. Effects of different culture protocols on the expression of discrete T cell receptor variable regions in human tumor infiltrating lymphocytes. Eur. J. Cancer 1993, 29A, 1754.
  3. L Filgueira, V Caetano, U Lüscher, A Juretic, M Zuber, F Harder, G Garotta, M Heberer, GC Spagnoli.. Differential effects of interleukin-2 and CD3 triggering on cytokine gene transcription and secretion in cultured human infiltrating lymphocytes. Cell. Immunol. 1993,150, 205.
  4. L Filgueira, FO Nestlé, M Rittig, HI Joller, P Groscurth. Human dendritic cells phagocytose and process Borrelia burgdorferi. J. Immunol. 1996, 157, 2998.
  5. G Grouard, I Durand, L Filgueira, J Banchereau, A-J Liu. Dendritic cells capable of stimulating T cells in germinal centres. Nature 1996, 383,364.
  6. G Grouard, M-C Rissoan, L Filgueira, I Durand, J Banchereau, Y-J Liu. The enigmatic plasmocytoid T cells deveop into dendritic cells with IL-3 and CD40-ligand. J. Exp. Med. 1997, 185, 1101.
  7. FO Nestlé, L Filgueira, BJ Nickoloff, G Burg. Human dermal dendritic cells process and present soluble protein antigens. J. Investig. Dermatol. 1998, 110, 762.
  8. Ch Beermann, G Lochnit, R Geyer, P Groscurth, L Filgueira. The lipid-component of lipoproteins from Borrelia burgdorferi: structural analysis, antigenicity and presentation via human dendritic cells. Biochem. Biophys. Res. Commun. 2000, 267, 897.
  9. Ch Beermann, H Wunderli-Allenspach, P. Groscurth, L Filgueira. Lipoproteins from Borrelia burgdorferi applied in liposomes and presented by dendritic cells induce CD8+ T-lymphocytes in vitro Cell. Immunol. 2000, 201, 124-131.
  10. SJ Wu, G Grouard, JR Mascola, R Putvana, MK Louder, L Filgueira, M Marovich, E Brachtel, G Murphy, N Bhamapravati, DL Birx, C Hayes, S Schlesinger Frankel. Human skin Langerhans cells are targets in transmission of dengue virus infection. Nature Medicine 2000, 6, 816-820.
  11. R Nunez, L Filgueira. Flow cytometric assessment of the reactivity of a panel of monoclonal antibodies (mAb) against two populations of human dendritic cells (DC). BMC Immunology 2001, 2:6
  12. E Eppler, H Horig, HL Kaufman, P Groscurth, L Filgueira. Carcinoembryonic antigen (CEA) presentation and specific T cell-priming by human dendritic cells transfected with CEA-mRNA. European Journal of Cancer 2002, 38, 184-193.
  13. L Filgueira. Fluorescence-based staining for tartrate-resistant acidic phosphatase (TRAP) in osteoclasts combined with other fluorescent dyes and protocols. J. Histochem. Cytochem. 2004, 52, 411.


Infrastructure for processing of biological samples for light and electron microscopy.